Solubilization and Humanization of Paraoxonase-1

Table 2

Activity of hum-4E9. k_cat/K_m values were determined under constant 2% methanol conditions for all three substrates. None of the enzymes could be saturated with those substrates under experimental conditions. S_P-CMP specific activities were determined at ~0.0125 mM S_P-CMP. GF k_app hydrolysis rate constants and stereoisomeric preferences were determined using GF at 0.315 mM. ND: not determined.

	paraoxon	EMP	CMP	SP-CMP	GF
Variant	kcat/Km; M^−1 s^−1	kcat/Km; M^−1 s^−1	kcat/Km; M^−1 s^−1	specific activity nmol min^−1 mg^−1	kapp; M^−1 s^−1	RP : SP ratio
4E9	3,100±200	24,000±1,000	17,000±3,000	1.1±0.1	ND	ND
hum-4E9	750±20	7,500±600	3,200±300	1.10±0.05	8,700	3.5 : 1
MBP-hum-4E9	230±80	6,800±300	6,000±2000	ND	5,400	6 : 1