Research Article

Supercritical SC-CO2 and Soxhlet n-Hexane Extract of Tunisian Opuntia ficus indica Seeds and Fatty Acids Analysis

Table 1

Relative content and results of GC-FID analysis of seeds oil fatty acid extracted by SC-CO2 and soxhlet n-hexane of two Tunisian O. ficus indica forms.

Tunisian O. ficus indica seedsRelative content [%] M ± SD
SC-CO2 extract ( 𝑇 = 4 0 °C, 𝑃 = 1 8 0 bar, CO2
flow rate = 15 mL·s−1 and time = 135 min)
Soxhlet n-hexane extract
Fatty acidsForms
SpinyThornlessSpinyThornless

Extraction yield 3 , 4 0 ± 0 , 1 4 1 , 9 4 ± 0 , 0 9 1 0 , 3 2 ± 0 , 0 9 8 , 9 1 ± 0 , 0 7
Myristic C14 : 0 0 , 1 9 ± 0 , 0 2 0 , 1 3 ± 0 , 0 2 0 , 1 1 ± 0 , 0 1 0 , 0 7 ± 0 , 0 1
Palmitic C16 : 0 1 4 , 3 0 ± 0 , 2 4 1 2 , 9 2 ± 0 , 4 5 1 1 , 3 3 ± 0 , 6 7 1 3 , 0 8 ± 0 , 8 8
Palmitoleic C16 : 1 0 , 9 7 ± 0 , 0 3 0 , 8 5 ± 0 , 0 6 0 , 6 6 ± 0 , 0 3 0 , 6 0 ± 0 , 0 1
Stearic C18 : 0 3 , 1 2 ± 0 , 0 4 2 , 3 8 ± 0 , 0 9 4 , 2 7 ± 0 , 2 6 4 , 0 9 ± 0 , 2 4
Oleic C18 : 1 2 2 , 3 1 ± 0 , 2 4 2 2 , 4 0 ± 0 , 5 3 2 5 , 2 8 ± 1 , 0 8 2 0 , 5 8 ± 0 , 4 2
Linoleic C18 : 2n6 5 7 , 6 0 ± 0 , 2 5 5 9 , 9 8 ± 0 , 2 2 5 7 , 5 4 ± 0 , 4 0 6 0 , 6 6 ± 0 , 7 3
Linolenic C18 : 3n3 0 , 2 3 ± 0 , 0 1 0 , 2 6 ± 0 , 0 3 0 , 4 5 ± 0 , 0 4 0 , 5 5 ± 0 , 0 1
Arachidic C20 : 0 0 , 3 7 ± 0 , 0 5 0 , 2 6 ± 0 , 0 3 0 , 3 6 ± 0 , 0 1 0 , 3 8 ± 0 , 0 5
Eicosenoic C20 : 1 0 , 3 2 ± 0 , 0 3 0 , 3 0 ± 0 , 0 2 ndnd
Eicosadienoic C20 : 2 0 , 2 0 ± 0 , 0 3 0 , 2 1 ± 0 , 0 2 ndnd
Behinic C22 : 0 0 , 3 9 ± 0 , 0 4 0 , 3 1 ± 0 , 0 4 ndnd
U/S4,445,255,224,68

M: mean ( 𝑛 = 3 ); SD: relative standard deviation; U/S: Unsaturation ratio, U/S = (C16 : 1 + C18 : 1 + C18 : 2n6 + C18 : 3n3 + C20 : 1 + C20 : 2)/(C14 : 0 + C16 : 0 + C18 : 0 + C20 : 0 + C22 : 0); nd: not detected.