High-Density Lipoprotein Binds to Mycobacterium avium and Affects the Infection of THP-1 Macrophages

Figure 5

Effects of HDL on the viability of M. avium engulfed by THP-1 macrophages. (a) THP-1 macrophages (10⁶/well) were infected with live M. avium in the presence or absence (control) of HDL (50 μg protein/mL) for 24 h. M. avium was recovered from lysed THP-1 macrophages for an assay of colony-forming units (CFUs; 3 weeks culture). (b) A part of infected THP-1 macrophages was further cultured for 24 and 48 h in a fresh medium. CFUs were also determined as described above. The data were indicated as the percentages against CFUs obtained without further cultivation. The values were indicated by mean ± SEM (, ).

(a)

(b)