Research Article

A Transcriptional Regulatory System of the S. cerevisiae OLE1 Gene Responds to Fatty Acid Species and Intracellular Amount, and not Simply Membrane Status

Table 1

Correlation of physical and structural properties of the fatty acids used in this study with regulatory effect on OLE1. For most fatty acids, the melting points were as reported [35]. The melting point of cis-10-heptadecenoic acid was determined as described in Section 2.

CompoundNumber of carbonsMW (daltons)Number/type of double bonds in acyl chainPosition of bond(s) from carboxy endMelting point (°C)Ability to regulate OLE1 expression (reporter gene assay)

cis-9 hexadecenoic acid (16:1∆9)162541/cis9–10−0.1°+++

cis-9 octadecenoic acid (18:1∆9)182821/cis9–1013.4°++

cis,cis-9,12 octadecadienoic acid (18:2∆9,12)182802/cis9–10, 12-13−12°+++

cis-10 heptadecenoic acid (17:1∆10)172681/cis10–1115.7–15.9°++

Hexadecanoic acid (16:0)162560NA145°0

trans-9 hexadecenoic acid (16:1∆9 trans)162541/trans9–1033–34°+

Octadecanoic acid (18:0)182840NA67°0