Detection of Bioactive Compounds in the Mucus Nets of Dendropoma maxima, Sowerby 1825 (Prosobranch Gastropod Vermetidae, Mollusca)
Figure 3
Digital detection of the inverted (grey-scale) bioluminescence signal of respective tracks. Each extract was made of ~5 nets educed in 1 mL ethyl acetate. M1–M5: bioactive metabolites with decreasing polarity. Lines bright coloured = nets with plankton sampled in situ, lines light coloured = nets without plankton sampled ex situ. Concentrations on plate: blue: 80 μL/band, red: 60 μL/band, green: 40 μL/band, and yellow: 20 μL/band. Due to equal intensities of M4 and M5 and the inverse ratio of M1/M4 and M1/M5 in both extracts (in situ: M4 and M5 < M1, ex situ: M4 and M5 > M1) of same concentration, M4 and M5 seem to exclusively be synthesised or accumulated by the wormsnail itself whereas M1 is of planktonic origin. .
