Divalent Metal- and High Mobility Group N Protein-Dependent Nucleosome Stability and Conformation

Figure 2

HMGN association with nucleosomal array is capable of inducing the S2 transition. Gel labels indicate HMGN identity, (N1t, HMGN1t; N1, HMGN1; N2, HMGN2; a, 12-array alone) and numbers designate HMGN : nucleosome molar stoichiometry. (a) HMGN binding to 12-array at low (top) and near physiological ionic strength (bottom). ((b), (c)) HMGN binding to 12-array at near physiological ionic strength with 1 mM Mg²⁺.

(a)

(b)

(c)