Formation, Repair, and Genotoxic Properties of Bulky DNA Adducts Formed from Tobacco-Specific Nitrosamines

Table 2

Comparative DNA adduct levels in lung and liver of F344 rats treated with 10 ppm NNK in the drinking water and sacrificed at various intervals [57, 58].

	Adduct Levels fmol/mg DNA (mean±S.D.)
Lung	Week	1	2	5	10	16	20
	𝑂^6-mG	976±342	1020±423	2550±263	1020±314	729±57.5	1910±615
	𝑂^6-pobdG	45±7^a	50±5^a	46±13^a	44±14^a	34±17^a	20±5^a
	7-pobG	750±95	1180±131	1360±214^a	2220±864	1700±175^a	1060±169
	𝑂^2-pobdT	1080±99	2020±150	3890±648	8260±2730^a	6720±606^a	5070±1060^a
	𝑂^2-pobC	240±23	250±18	400±87^a	730±211	810±152	940±175
Liver	Week	1	2	5	10	16	20
	𝑂^6-mG	3830±865	7120±2080	2310±946	564±250	637±59	891±379
	𝑂^6-pobdG	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
	7-pobG	490±104^a	880±182^a	1050±90	1460±625	1170±86^a	730±225
	𝑂^2-pobdT	650±121^a	1230±272^a	2190±174	3740±1170^a	3540±643^a	2680±643^a
	𝑂^2-pobC	170±43^a	140±25^a	240±17	580±214	350±152	490±146

n.d., not detected.
aSignificantly different from 𝑂⁶-mG, 𝑃<.05.