Effect of N3-Methyladenine and an Isosteric Stable Analogue on DNA Polymerization
Figure 1
The A4 tract sequence is methylated selectively by Me-lex at A-27 and A-26. Maxam-Gilbert chemical sequencing of methylation pattern in -[32P]-labeled unmodified sequence (Table 1) by Me-lex at 4°C and 20°C with 12 or 24 hours incubation times in the presence of: the 10 nt duplex forming oligomer (DFO), which overlaps with underlined bases on the labeled strand, with 0.5, 1.25 or 2.5 mM Me-lex (12 hours) or 0 or 2.5 mM Me-lex (24 hours); the full complementary strand with 0, 1.25 or 2.5 mM Me-lex (12 hours) or 0 or 2.5 mM Me-lex (24 hours); no complementary strand (none) with 0 or 2.5 mM Me-lex (24 hours). Sequencing lanes (G, ladder and C) are on right.
