Effect of N3-Methyladenine and an Isosteric Stable Analogue on DNA Polymerization
Figure 7
The effect of the position of the c3A and 3-m-c3A lesions within the A4 tract on the insertion by T7 Sequenase opposite the modification using 10 or 100โM of the dNTPs. The location of the modification is denoted by the numbering system in Scheme 1. The primers (Table 1, P23, P24, P25 and P26) stop 1โnt before the position of the c3A or 3-m-c3A modification.