Research Article

Effect of N3-Methyladenine and an Isosteric Stable Analogue on DNA Polymerization

Figure 7

The effect of the position of the c3A and 3-m-c3A lesions within the A4 tract on the insertion by T7 Sequenase opposite the modification using 10 or 100โ€‰ ๐œ‡ M of the dNTPs. The location of the modification is denoted by the numbering system in Scheme 1. The primers (Table 1, P23, P24, P25 and P26) stop 1โ€‰nt before the position of the c3A or 3-m-c3A modification.
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