426505.fig.008a
(a)
426505.fig.008b
(b)
Figure 8: Extension from (A) c3A N and (B) 3-m-c3A N by T7 Sequenase. The template strand containing a c3A or 3-m-c3A at A-27 was annealed to a 5 -Cy5-primer (Table 1, P27). T7 Sequenase was added and the reactions initiated by the addition of the individual dNTP’s. Extension is most efficient from c3A T termini, while extension is similar from the 3-m-c3A T and 3-m-c3A G. Note that for 3-m-c3A G only one dGTP is added rather than the two observed for 3-m-c3A T.