Figure 8: Extension from (A) c3AN and (B) 3-m-c3AN by T7 Sequenase. The template strand containing a c3A or 3-m-c3A at A-27 was annealed to a -Cy5-primer (Table 1, P27). T7 Sequenase was added and the reactions initiated by the addition of the individual dNTP’s. Extension is most efficient from c3AT termini, while extension is similar from the 3-m-c3AT and 3-m-c3AG. Note that for 3-m-c3AG only one dGTP is added rather than the two observed for 3-m-c3AT.