Effect of N3-Methyladenine and an Isosteric Stable Analogue on DNA Polymerization

Table 1

Sequences of oligomers and primers used in the studies.

Oligomer name	Sequence	Length	Modification
Primers
P20	5^ CCG TAG GAC TGC CGT AGT GC 3^	20 nt	5^-Cy5
P23	5^ CCG TAG GAC TGC CGT AGT GCT GG 3^	23 nt	5^-Cy5
P24	5^ CCG TAG GAC TGC CGT AGT GCT GGT 3^	24 nt	5^-Cy5
P25	5^ CCG TAG GAC TGC CGT AGT GCT GGT T 3^	25 nt	5^-Cy5
P26	5^ CCG TAG GAC TGC CGT AGT GCT GGT TT 3^	26 nt	5^-Cy5
P27	5^ CCG TAG GAC TGC CGT AGT GCT GGT TTN 3^ (N = C, G, T or A)	27 nt	5^-Cy5
Templates
Unmodified	5^ ATC CAA CCT GTA TCA TAC TTA CCA AAA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	—
3-m-c^3A/24	5^ ATC CAA CCT GTA TCA TAC TTA CCA AAXCCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = 3-m-c^3A
3-m-c^3A/25	5^ ATC CAA CCT GTA TCA TAC TTA CCA AXA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = 3-m-c^3A
3-m-c^3A/26	5^ ATC CAA CCT GTA TCA TAC TTA CCA XAA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = 3-m-c^3A
3-m-c^3A/27	5^ ATC CAA CCT GTA TCA TAC TTA CCX AAA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = 3-m-c^3A
c^3A/24	5^ ATC CAA CCT GTA TCA TAC TTA CCA AAX CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = c^3A
c^3A/25	5^ ATC CAA CCT GTA TCA TAC TTA CCA AXA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = c^3A
c^3A/26	5^ ATC CAA CCT GTA TCA TAC TTA CCA XAA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = c^3A
c^3A /27	5^ ATC CAA CCT GTA TCA TAC TTA CCX AAA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = c^3A
THF/24	5^ ATC CAA CCT GTA TCA TAC TTA CCA AAX CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = THF
THF/25	5^ ATC CAA CCT GTA TCA TAC TTA CCA AXA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = THF
THF/26	5^ ATC CAA CCT GTA TCA TAC TTA CCA XAA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = THF
THF/27	5^ ATC CAA CCT GTA TCA TAC TTA CCX AAA CCA GCA CTA CGG CAG TCC TAC GG 3^	50 nt	X = THF
DFO	5^-TGGTTTTGGT	10 nt