Effect of Base Sequence on G-Wire Formation in Solution

Table 2

Hydrodynamic parameters obtained from DLS measurements in dialysed samples at 0.4 mM DNA concentration and room temperature. The length of the quadruplex 𝐿 is estimated from (6) using 𝑑=2.6 nm for the quadruplex diameter [26, 39]. For comparison, the length of extended oligonucleotides 𝐿_oligo is estimated by multiplying the number of bases with 0.34 nm, the average base distance.

Designation	𝐷𝑓 (10^10 m^2/s)	𝑟𝐻 (nm)	Length (nm)	𝐿oligo (nm)
Sequence 1	1.06 ± 0.037	2.26 ± 0.034	6.1 ± 0.5	6.8
Sequence 2	—	—	—	3.1
Sequence 3	3.0 ± 0.30	0.78 ± 0.08	—	3.1
Sequence 4	0.89 ± 0.040	2.69 ± 0.044	8.8 ± 0.8	4.8
Sequence 5	0.79 ± 0.008	3.03 ± 0.062	11.0 ± 0.2	9.9
Sequence 6	1.15 ± 0.041	2.09 ± 0.036	5.0 ± 0.4	6.5