Structural Determinants of Photoreactivity of Triplex Forming Oligonucleotides Conjugated to Psoralens
Figure 5
Photoreactivity of TFOs conjugated to HMT. 50 nM TFO DNA-C2 or TFO RNA-C6 were incubated with 5 nM duplex target overnight and then irradiated with varying doses of UVA. (a) Separation of reaction products by polyacrylamide gel electrophoresis under denaturing conditions. The products are marked as unreacted (U), monoadducts (MA), and crosslinks (XL). Quantitative analysis (b) TFO DNA-C2 and (c) TFO RNA-C6 for unreacted DNA (circles), MA (squares), and XL (triangles).
