Role of Topoisomerase II in DNA Damage Response following IR and Etoposide
Figure 6
TARDIS analysis of cells treated with etoposide continuously for 24 hours plus and minus MG132 or with MG132 alone. Topoisomerase II-DNA adducts were quantified using an antibody that detects both isoforms. WT MEFs following exposure to 1 M 2 M MG132 (a), Top2 MEFs following exposure to 1 M 2 M MG132 (b), WT MEFs following exposure to 10 M 2 M MG132 (c), Top2 MEFs following exposure to 10 M 2 M MG132 (d), or WT and TOP2 MEFs following exposure to 2 M MG132 alone (e). Plots show the mean of the median FITC fluorescence.