The Role of PCNA Posttranslational Modifications in Translesion Synthesis

Figure 1

Fork stalling results in monoubiquitylation of the replication clamp PCNA which increases its affinity to a TLS polymerase. The latter replaces the high-fidelity replicative polymerase and it can accommodate the lesion even with the risk of generating mutations in certain circumstances. This Polymerase binds PCNA through its PIP box and a ubiquitin binding domain.