Conformational-Dependent and Independent RNA Binding to the Fragile X Mental Retardation Protein
Figure 6
Interaction of FMRP with the 5′ and 3′ ends of BC1 RNA. (a) 35S-FMRP was incubated with 2 μM annealed full-length BC1 RNA, or annealed versions of the first 75 b of BC1 RNA (5′BC1-75), the first 60 b of BC1 RNA (5’BC1-60) the last 60 b of BC1 RNA (3′BC1-60) and an 85 b transcript containing the HIV1 TAR hairpin. The percent binding corrected for background of 6 reactions per protein is plotted. (b) Secondary structure model of 5′BC1-60 RNA from M-fold. (c) Secondary structure model of the HIV1 TAR RNA from M-fold; the 27 b leader sequence has no effect upon the folding.
