A Structurally Variable Hinged Tetrahedron Framework from DNA Origami

Figure 2

(Left) Transmission electron microscopy (TEM) imaging of tetrahedron structure containing all staple nucleotides. Typically, tetrahedrons displayed one or more ruptured struts, generating the widely observed flattened configuration. Scale bars: 60 nm. (Right) Agarose gel electrophoresis separation of the different configurations formed by targeted gap staple omission in struts 1, 5, and 6. Left to right: lanes 1-2 contain a 1 kb ladder and the circular p8634 scaffold, respectively. Lanes 3–8 contain the full tetrahedron, and the open configurations n156, n15, n16, n56, and n6, as indicated by the sketched representations above each lane.