About this Journal Submit a Manuscript Table of Contents
Journal of Nucleic Acids
Volume 2011 (2011), Article ID 371517, 17 pages
http://dx.doi.org/10.4061/2011/371517
Research Article

A Genomic Approach to Study Anthocyanin Synthesis and Flower Pigmentation in Passionflowers

Departamento de Biologia Vegetal. Rua Monteiro Lobato 970, Instituto de Biologia, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz, 13083-970 Campinas, SP, Brazil

Received 14 January 2011; Accepted 1 March 2011

Academic Editor: Francisco Cruz-Sosa

Copyright © 2011 Lilian Cristina Baldon Aizza and Marcelo Carnier Dornelas. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Most of the plant pigments ranging from red to purple colors belong to the anthocyanin group of flavonoids. The flowers of plants belonging to the genus Passiflora (passionflowers) show a wide range of floral adaptations to diverse pollinating agents, including variation in the pigmentation of floral parts ranging from white to red and purple colors. Exploring a database of expressed sequence tags obtained from flower buds of two divergent Passiflora species, we obtained assembled sequences potentially corresponding to 15 different genes of the anthocyanin biosynthesis pathway in these species. The obtained sequences code for putative enzymes are involved in the production of flavonoid precursors, as well as those involved in the formation of particular (“decorated”) anthocyanin molecules. We also obtained sequences encoding regulatory factors that control the expression of structural genes and regulate the spatial and temporal accumulation of pigments. The identification of some of the putative Passiflora anthocyanin biosynthesis pathway genes provides novel resources for research on secondary metabolism in passionflowers, especially on the elucidation of the processes involved in floral pigmentation, which will allow future studies on the role of pigmentation in pollinator preferences in a molecular level.