Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA

Figure 2

Specific chemical reactions for m³C and m⁵C detection. (a) Cleavage of m³C and unmodified uridine by hydrazine. (b) Deamination of unmodified cytosine by bisulfate; m⁵C is resistant for deamination. (c) Specific oxydation of 5-6 double C=C bond in methylated pyrimidines by MnO₄⁻, OsO₄, and OsO₄ derivatives.

(a)

(b)

(c)