Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA

Figure 6

Examples of thiolated nucleotides found in RNA (a) and (N-acryloylamino)phenyl-4-mercuric chloride (APM) which is used for gel-retardation detection of thiolated molecules. (b) Reaction of thiolated nucleotides with iodoacetamide and its derivatives. s²U is taken to illustrate the reaction product.

(a)

(b)