Use of Specific Chemical Reagents for Detection of Modified Nucleotides in RNA
Figure 9
Reaction of guanosine residues in RNA with glyoxal and stabilization of the resulting adduct by boric acid H3BO3. Inosine residues do not form the stable product under similar conditions (a). Organic derivative of boric acid (N-acryloylamino)phenyl-3-boronic acid (APB) and its complex with cisdiols present in queosine and the dialdehyde resulting from periodate oxydation of the cisdiol (b).
