Coupling Strategies for the Synthesis of Peptide-Oligonucleotide Conjugates for Patterned Synthetic Biomineralization

Table 1

Reaction yields for POC formation in the listed conditions.

Reaction	Coupling reagent	Solvent	Supporting media	Temperature	Reaction time	Yield (%)
1	HBTU	DMF	Sepharose	room temperature	16 h	0
2	PyBOP	DMF	Sepharose	room temperature	16 h	4
3	DMTMM	Water	Sepharose	room temperature	16 h	15
4	EDC	Water	Sepharose	room temperature	16 h	24
5	DMTMM	Water	Sepharose	50°C	16 h	15
6	DMTMM	Water	Sepharose	room temperature	2 h	15
7	PyBOP	DMF	Sepharose	room temperature	2 h	4
8	DMTMM	Water	Solution	room temperature	16 h	50
9	EDC	Water	Solution	room temperature	16 h	95
10	DMTMM	Water	Solution	50°C	16 h	55