Evaluation of Fluorescent Analogs of Deoxycytidine for Monitoring DNA Transitions from Duplex to Functional Structures

Table 1

The 113-mer DNA sequence that is part of the cruciform found in the terminator of the Amp^R cassette in the cloning vector pBR322 under superhelical strain. The location of the substitution of cytidine by the fluorescent analogs marked by an asterisk.

Sequence
5′-TGA GGT TAA GGG ATT TTG GTC ATG AGA TTA TCA AAA AGG ATC TTC* ACC TAG ATC CTT TTA ATT AAA AAT GAA GTT TTA AAT CAA TCT AAA GTA TAT ATG AGT AAA CTT GGG C -3′