Metal Ion Chelates as Surrogates of Nucleobases for the Recognition of Nucleic Acid Sequences: The Pd2+ Complex of 2,6-Bis(3,5-dimethylpyrazol-1-yl)purine Riboside

Table 1

The sequences, RP-HPLC retention times, wavelengths of UV absorption maxima, and observed and calculated molecular weights of the oligonucleotides prepared.

Sequence		𝑡R /minute^a	λ/nm	m/z obsd.	m/z calcd.
7X	5′-GCGCXCCGG-3^′b	16.0	267.8	3152.8	3152.7
7A	5′-GCGCACCGG-3′	16.1	257.6	2993.6	2993.8
8U	3′-CGCGUGGCC-5′	17.2	258.4	2970.6	2970.8
8A	3′-CGCGAGGCC-5′	17.5	257.6	2993.6	2993.8
8G	3′-CGCGGGGCC-5′	14.1	257.2	3009.6	3009.8
8C	3′-CGCGCGGCC-5′	16.6	258.8	2969.6	2969.8
9U	3′-CGCAUAGCC-5′	11.8	260.2	2938.6	2938.8
9A	3′-CGCAAAGCC-5′	12.3	259.1	2961.6	2961.8
9G	3′-CGCAGAGCC-5′	11.9	261.0	2977.6	2977.8
9C	3′-CGCACAGCC-5′	12.4	267.0	2937.6	2937.8
10T	3′-d(CGCGTGGCC)-5′	10.8	258.0	2714.3	2714.8
10A	3′-d(CGCGAGGCC)-5′	10.0	257.2	2723.4	2723.8
10C	3′-d(CGCGCGGCC)-5′	11.0	271.2	2699.3	2699.8
10G	3′-d(CGCGGGGCC)-5′	10.5	256.0	2739.4	2739.8

^aSee Section 5 for the HPLC conditions. ^bX refers to the artificial nucleoside 1.