Dioxaphosphorinane-Constrained Nucleic Acid Dinucleotides as Tools for Structural Tuning of Nucleic Acids
Table 2
Sequences and melting temperatures of CNAgm containing duplexes.
Entry
Sequencea
(°C)
(°C)
1
5′-GCGTTTTTTGCT-3′ 3′-CGCAAAAAACGA-5′
49.0
—
2
5′-GCGTTTxT TTGCT-3′ 3′-CGCAAAAAACGA-5′
55.0
+6.0
3
5′-GCGTTTxTTxT GCT-3′ 3′-CGCAAAAAACGA-5′
59.0
+10.0
4
5′-GCGTxTTxTTxT GCT-3′ 3′-CGCAAAAAACGA-5′
64.0
+15.0
5
5′-GCAAAAACTTGC-3′ 3′-CGTTTTTGAACG-5′
48.0
—
6
5′-GCAAAAACTxT GC-3′ 3′-CGTTTTTGAACG-5′
53.2
+5.2
7
5′-GCAAAAACTTGC-3′ 3′-CGTTTTxT GAACG-5′
52.2
+4.2
8
5′-GCAAAAACTTGC-3′ 3′-CGTTTxT TGAACG-5′
54.3
+6.3
9
5′-GCAAAAACTTGC-3′ 3′-CGTTxT TTGAACG-5′
53.4
+5.4
10
5′-GCAAAAACTTGC-3′ 3′-CGTxT TTTGAACG-5′
52.4
+4.4
11
5′-GCAAAAACTxT GC-3′ 3′-CGTTTTxT GAACG-5′
55.9
+7.9
12
5′-GCAAAAACTxT GC-3′ 3′-CGTTTxT TGAACG-5′
56.4
+8.4
13
5′-GCAAAAACTxT GC-3′ 3′-CGTTxT TTGAACG-5′
57.6
+9.6
14
5′-GCAAAAACTxT GC-3′ 3′-CGTxT TTTGAACG-5′
57.8
+9.8
aTxT
: () -D-CNA TT (CNAgm) within the strand. bUV melting experiments were carried out in sodium phosphate buffer (10 mM, pH 7.0) containing NaCl (100 mM) and EDTA (1 mM).
