PCR Amplification and Transcription for Site-Specific Labeling of Large RNA Molecules by a Two-Unnatural-Base-Pair System

<table>Chemical structures of the Ds-Px, Ds-Pa′, and Ds-Pa pairs. The Ds-Px pair is employed for PCR amplification to prepare the Ds-containing DNA templates. The Ds-Pa′ pair is employed for DNA sequencing, to determine the Ds position in the DNA templates. The Ds-Pa pair is employed for T7 transcription, to incorporate Biotin-Pa into RNA opposite Ds in the templates. Px and Pa bases modified with various functional groups (R) can also be used as substrates for PCR and transcription, respectively. The unnatural substrates used in this study are summarized in the box.</table>

Journal of Nucleic Acids

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Figure 1

Figure 1: PCR Amplification and Transcription for Site-Specific Labeling of Large RNA Molecules by a Two-Unnatural-Base-Pair System 