UvrD Participation in Nucleotide Excision Repair Is Required for the Recovery of DNA Synthesis following UV-Induced Damage in <i>Escherichia coli</i>

<table>UvrD is required for the recovery of replication following UV irradiation, but not for replication in the absence of damage. Cells grown in the presence of [<sup>14</sup>C]-thymine were pulse-labeled for 2 min with [<sup>3</sup>H]-thymidine at the times indicated following either UV irradiation with 27 J/m<sup>2 </sup> (open symbols) or mock irradiation (closed symbols). Total DNA accumulation (<sup>14</sup>C incorporation, circles) and rate of synthesis (<sup>3</sup>H incorporation/ 2 min, squares) are plotted. Graphs represent the average of at least three independent experiments. Error bars represent the standard error of the mean. The level of [<sup>3</sup>H] and [<sup>14</sup>C] in preirradiated DNA ranged between 30,000–50,000 cpm and 3000–6000 cpm for all experiments.</table>

Journal of Nucleic Acids

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Figure 2

Figure 2: UvrD Participation in Nucleotide Excision Repair Is Required for the Recovery of DNA Synthesis following UV-Induced Damage in <i>Escherichia coli</i> 

Figure 2 | UvrD Participation in Nucleotide Excision Repair Is Required for the Recovery of DNA Synthesis following UV-Induced Damage in Escherichia coli 