Figure 4: Self-self hybridization at 64°C of 2 μg (per dye) of labeled total mouse brain RNA. Diagrams of average intensity values show filtered results in logarithmic scale. The axis represents values of AlexaFluor3 dye measurement at 532 nm, and the axis represents values of AlexaFluor5 dye measurement at 635 nm. Red spots represent all signals from LNA and DNA probes spotted on the microarray slide. (a) Detection of snoRNAs (yellow spots) with antisense DNA probes. (b) Detection of miR-9 (yellow spots, black arrows) and miR-9* (yellow spots, white arrow). Sense DNA probes were below detection levels and filtered out. (c) Diagram showing mean intensity values ( axis) of all antisense DNA probes detecting snoRNAs.