Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray
Figure 5
Self-self hybridization at 64°C of 2 μg (per dye) of labeled total mouse brain RNA. Diagrams of average intensity values show filtered results in logarithmic scale. The axis represents values of AlexaFluor3 dye measurement at 532 nm, and the axis represents values of AlexaFluor5 dye measurement at 635 nm. Red spots represent all signals from LNA and DNA probes spotted on the microarray slide. (a) Detection of snoZ39 (yellow spots) with antisense DNA probes (black arrow, blow up) and one nucleotide mismatch DNA probe (white arrow, blow up). (b) Diagram showing on the axis the percentage of mean detection value of the antisense probe SNOZ39−6–60MM1 and the one nucleotide mismatch probe SNOZ39−6–60MM1. (c) Diagram showing the mean intensity values ( axis) of the antisense probes of one (MM1) and two (MM2) nucleotide mismatch probes for 7SK RNA and SNORD55.
