MsDpo4—a DinB Homolog from Mycobacterium smegmatis—Is an Error-Prone DNA Polymerase That Can Promote G:T and T:G Mismatches

Figure 1

Sequence alignment of representative DinB homologs. The sequences of Dpo4 from Sulfolobus solfataricus (SsDpo4), Escherichia coli (EcCpo4), Mycobacterium tuberculosis (Rv3056), and Mycobacterium smegmatis (MsDpo4) are compared. Active site residues are displayed in blue color. The identical residues in Rv3056 and MsDpo4 are displayed with similar background colour. The different domains (palm, fingers, thumb, and PAD) observed in Y-family DNA polymerases are delineated on the basis of the ssDpo4 structure (1JX4). CBM represents the processivity clamp binding motif.