MsDpo4—a DinB Homolog from Mycobacterium smegmatis—Is an Error-Prone DNA Polymerase That Can Promote G:T and T:G Mismatches
Table 2
Frequency of dG:dTTP and dT:dGTP misincorporation by MsDpo4 as determined by steady-state analysis.
Template: incoming nucleotide
(M)
/(nM of enzyme−1 min−1)
dG:dCTP [4]
4.3 (0.69)
16.16 (5.6)
1
dG:dTTP [4]
165.5 (39.70)
dT:dATP [4]
5.5 (1.54)
12.74 (2.3)
1
dT:dGTP [4]
166.5 (39.01)
The nucleotide misincorporation ratio, . For example, = . Number of experimental trials are given in . Standard errors between the experiments are given in ().
