Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems

Table 2

Consignment services of phage display with in-house libraries.

Company name	Peptide design	Peptide structure
Creative Biolabs	X10, X16, or X20^*1	linear
Dyax	XaCXbCXc^*1	cyclic
Bicycle Therapeutics	XaCXbCXcCXd^*2	cyclic with a non-natural linker

X stands for any randomized amino acid.
^*1The library was built by varying 19 aa at the randomized positions; the codon encoding Cys is excluded.
^*2Bicyclic peptide library was made via thioether linkages [1].