Small RNA Expression Profiling by High-Throughput Sequencing: Implications of Enzymatic Manipulation
Figure 1
Enzymatic manipulation of RNAs with modifications at their 5′- or 3′-ends. Black lines represent RNA with the left and right ends representing the 5′- and 3′-ends, respectively. One, two, or three grey circles represent mono-, di-, or triphosphate at the 5′-end. “A” and “mG” represent a 3′ to 5′ AMP and cap structure at RNA 5′-end. “2′-OH” or “2′, 3′-OH” represents RNAs with no modification at the 3′-end. “2′-OCH3” and “2′, 3′-CP” represent 2′-O-methylation and 2′, 3′-cyclic phosphate at the 3′-end, respectively. Dashed lines represent degraded RNA. The nucleotide “N” in grey color represents the nucleotide removed during the β-elimination reaction. MthRnl, TAP, T4 PNK, and XRN1 are the abbreviations of Methanobacterium thermoautotrophicum RNA ligase, tobacco acid pyrophosphatase, T4 polynucleotide kinase, and a 5′-monophosphate-dependent 5′ to 3′exoribonuclease, respectively.
