Small RNA Expression Profiling by High-Throughput Sequencing: Implications of Enzymatic Manipulation

Figure 2

Small RNA high-throughput sequencing library construction methods. The 5′- and 3′-adapters are shown as blue and red lines, respectively. sRNAs are depicted as grey lines. After sRNAs are converted into DNA, the sequences are shown as black lines. The asterisks represent steps suitable for introducing barcodes in each method. The dashed lines with arrows illustrate cDNA synthesis. At the bottom of each schematic diagram, RNA 5′-end requirement and sensitivity to 2′-O-methyl modification at the 3′-end for each method are noted.