Chemical Approaches for Structure and Function of RNA in Postgenomic Era
Figure 16
Two-dimensional separation of alkali stable dinucleoside monophosphate [18, 39]. (a) Standard dinucleoside monophosphates (NmpN) were separated on Whatman no.1 paper with the solvent systems ethylacetate-1-propanol-water (4 : 1 : 2, v/v) in the first dimension and in the second dimension with 2-propanol-water-concentrated ammonium hydroxide (7 : 2 : 1, V/V). (b) Autoradiograph of two-dimensional separation of alkali-stable dinucleoside monophosphate of U2 RNA from Novikoff hepatoma cell nuclei. The [32P]-labeled U2 RNA was hydrolyzed by 0.3 N NaOH, and the sample was separated on a DEAE-Sephadex column A-25 at pH 7.6. The dinucleotides were collected and treated with alkaline phosphatase. The alkali stable dinucleoside monophosphates were separated on Whatman no. 1 paper and autoradiographed with X-ray film.
