In Vitro Selection of Fab Fragments by mRNA Display and Gene-Linking Emulsion PCR

Table 1

Oligonucleotide sequences.

Name	Sequence (5′ to 3′)
Universal-OL1	GAAATTAATACGACTCACTATAGGGAAACCACTACGGTCTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG; GAGATATACCA
Myc-R	AAGGTCTTCTTCACTAATCAGTTTCTGCTC
Universal-OL2	GAAATTAATACGACTCACTATAGGGAGACCGTAGTGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAG; GAGATATACCA
FLAG-R	CTTGTCGTCATCGTCCTTGTAGTC
Myc25A-R	TTTTTTTTTTTTTTTTTTTTTTTTTAAGGTCTTCTTCACTAATCAGTTTCTGCTC
FLAG25A-R	TTTTTTTTTTTTTTTTTTTTTTTTTCTTGTCGTCATCGTCCTTGTAGTC
PolyT oligo	TTTTTTTTTTTTTTTTTTTTTTTTT
UnivOL1-F	GAAATTAATACGACTCACTATAGGGAAACCACTACGGTC
UnivOL2-F	GAAATTAATACGACTCACTATAGGGAGACCGTAGTGGTT
Fluo-F	CAGGATCGAGTGGGTCAAAC
Fluo-R	GGTGCTGGAGCTTTTGTCTG
p53-F	GGCAGAGCTTGTAAGGTCAG
p53-R	CAATCCATCCAATCCACTCC

The bold type resembles the sequences of the overlapping region for overlap-extension PCR.
Poly T oligo is labeled with a photo-cleavable biotin at the 5′ end.