Synthetic Nucleotides as Probes of DNA Polymerase Specificity
Figure 5
Taq polymerase activity is severely inhibited by (a) 5-(2′-Deoxy-β-D-ribofuranosyl)-3-methyl-2-pyridone-5′-triphosphate d*TTP and (b) 2-Amino-5-(2′-deoxy-β-D-ribofuranosyl)pyridine-5′-triphosphate (d*CTP), dC, and dT analogs, respectively, lacking the 2-keto groups [87].
