Genetically Encoded Libraries of Nonstandard Peptides
Figure 1
Generation of genetically encoded libraries of nonstandard peptides. (a) Genetic code expansion. Nonproteinogenic amino acids are assigned to the amber codon and four-base codons. (b) Reprogramming the genetic code. Nonproteinogenic amino acids are reassigned to blank codons generated by reconstructing a cell-free translation system with a reduced number of amino acids and protein factors. (–9) represents one of the nonproteinogenic amino acids.
