Journal of Nucleic Acids / 2012 / Article / Tab 2 / Review Article
Challenges and Opportunities for Small Molecule Aptamer Development Table 2 A listing of DNA aptamers reported in the open literature* (up until July 2012) that have been confirmed to bind to small molecule targets. The dissociation constant (
), a measure of binding affinity, is included as well as the year of aptamer development.
Target Binding affinity (
) Year Reference Reactive green 19 33 μ M 1992 [130 ] Adenosine monophosphate and adenosine triphosphate 6 μ M 1995 [131 ] L-arginine 2.5 mM 1995 [132 ] L-argininamide 0.25 mM 1995 [132 ] Anionic porphyrins 0.4–4.9 μ M 1996 [88 ] Sulforhodamine B 190 nM 1998 [61 ] Cellobiose 600 nM 1998 [133 ] 7,8-dihydro-8-hydroxy-2′-deoxyguanosine 270 nM 1998 [134 ] Cholic acid 5–67.5 μ M 2000 [135 ] Hematoporphyrin 1.6 μ M 2000 [136 ] L-tyrosinamide 4.5 μ M 2001 [137 ] Sialyllactose 4.9 μ M 2004 [138 ] Ethanolamine 6–19 nM 2005 [139 ] (R)-thalidomide 1 μ M 2007 [49 ] Hoechst derivative 7e 878 nM 2007 [140 ] 17β -estradiol 0.13 μ M 2007 [141 ] Lys-Arg-Azobenzene-Arg 0.33 μ M 2007 [142 ] Tetracycline 64 nM 2008 [143 ] L and D arginine 580–810 μ M 2008 [144 ] Daunomycin 10 nM 2008 [145 ] Oxytetracycline 10 nM 2008 [54 ] Ochratoxin A 200 nM 2008 [26 ] Dopamine 700 nM 2009 [146 ] 8-hydroxy-2′-deoxyguanosine 100 nM 2009 [147 ] Diclofenac 42.7–166.34 nM 2009 [148 ] (S) and (R)-ibuprofen 1.5–5.2 μ M 2010 [50 ] Adenosine triphosphate 3.7 μ M 2010 [31 ] Fumonisin B1 (FB1 ) 100 nM 2010 [7 ] Acetamiprid 4.98 μ M 2011 [149 ] Kanamycin 78.8 nM 2011 [150 ] L-tryptophan 1.757 μ M 2011 [151 ] Bisphenol A 8.3 nM 2011 [53 ] Ochratoxin A 96–293 nM 2011 [152 ] Phenylphosphonic dichloride >50 μ M 2011 [153 ] Organophosphorus pesticides (phorate, profenofos, isocarbophos and omethoate) 0.8–2.5 μ M 2012 [154 ] Polychlorinated biphenyls (PCB77) 4.02, 8.32 μ M 2012 [155 ] Polychlorinated biphenyls (PCB72 and PCB106) 60–100 nM 2012 [156 ] Ampicillin 9.4–13.4 nM 2012 [157 ]
Only aptamer sequences that have experimentally determined
values were included in this table.