Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain
Figure 6
The splicing silencing roles of SC35 and ASF/SF2 are differentially affected by the deletion of cognate motifs in the downstream intron. (a) Schematic indicates structure of parent reporter, CASK_E24a, and derivatives, del1 and del2. The deleted sequences are shown above and below the schematic, where nucleotide positions specify the boundaries of each deletion relative to the beginning of the intron. SC35 motifs, black ovals; ASF/SF2, grey ovals. The copy number of motifs in each deleted region is indicated in parentheses. (b) Gel panel displays exon included and skipped products measured by RT-PCR from N18TG2 transfections. Splicing reporters were cotransfected with ASF/SF2 (lanes 2, 5, 8) or SC35 (lanes 3, 6, 9) expression vector. Control samples (ctrl) received empty vector plasmid (lanes 1, 4, 7). (c) Bar graph illustrates the % exon inclusion values for the gel panel above. Error bars represent standard deviations.
