Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes

Figure 6

Flow chart for protein screening using liposome-based IVC. Library of mutated genes are compartmentalized with PURE system and other reagents into liposome. Each protein variant is synthesized from a single copy of gene in each liposome. Liposomes encapsulating the gene of interest are screened using an FACS. DNA extracted from the liposomes is amplified to be transferred to the next round of gene screening.