Imaging mRNA Expression in Live Cells via PNA·DNA Strand Displacement-Activated Probes
Figure 5
Fluorescence activation of the strand displacement probes by iNOS mRNA.(a) Fluorescence spectra of the iNOS probe (0.2 M iNOS-FAM-PNA annealed to 0.4 M iNOS-DNA-DABCYL) in the presence of 0.1, 0.2, and 0.4 M iNOS mRNA. Positive control: 0.2 M iNOS-FAM-PNA annealed to 0.4 M iNOS-DNA. (b) Fluorescence spectra of the pLuc probe (0.2 M FAM-pLuc-PNA annealed to 0.4 M pLuc-DNA-DABCYL) in the presence of 0.4 M iNOS mRNA. Excitation wavelength 488 nm. Temperature: 37°C.
