Imaging mRNA Expression in Live Cells via PNA·DNA Strand Displacement-Activated Probes

Figure 6

Kinetics of fluorescence activation by strand-displacement probes by iNOS mRNA. Fluorescence emission at 525 nm of the iNOS probe (0.05 𝜇M FAM-iNOS-PNA annealed to 0.1 𝜇M iNOS-DNA-DABCYL) or pLuc probe (0.05 𝜇M FAM-pLuc-PNA annealed to 0.1 𝜇M pLuc-DNA-DABCYL) with the indicated amount of iNOS mRNA. Excitation wavelength: 488 nm, temperature: 37°C.