Imaging mRNA Expression in Live Cells via PNA·DNA Strand Displacement-Activated Probes
Figure 7
Live cell imaging of iNOS mRNA expression with the strand-displacement probes. Z-stack projection of confocal fluorescent images of RAW 264.7 cells and the quantitative analysis of fluorescence in selected regions of interests (ROIs) 24 h after transfection. The iNOS probe (0.4 M FAM-iNOS-PNA annealed to 0.5 M iNOS-DNA-DABCYL) or pLuc probe (0.4 M FAM-pLuc-PNA annealed to 0.5 M pLuc-DNA-DABCYL) was delivered with 9.7 g/mL cSCK nanoparticle at an N/P ratio of 8 : 1. Green: FAM signal. Blue: Hoechst nuclear stain.
