Allelic Imbalance in TOR1A mRNA Expression in Manifesting and Non-Manifesting Carriers of the GAG-Deletion

Table 1

Affinity of allele-specific primers for the wildtype (WT) and the ΔGAG allele of TOR1A.

DNA template	Primers	Cp	ΔCp
1(a) WT/WT	AS-F	22.34	−7.21
	ASWT-R
1(b) WT/WT	AS-F	29.55
	ASΔE-R
2(a) WT/ΔGAG	AS-F	23.33	0.20
	ASWT-R
2(b) WT/ΔGAG	AS-F	23.13
	ASΔE-R
3(a) ΔGAG	AS-F	19.47	11.87
	ASWT-R
3(b) ΔGAG	AS-F	7.60
	ASΔE-R

WT/WT-homozygous for the normal allele; WT/ΔGAG-TOR1A carrier heterozygous for the mutant allele; ΔGAG-plasmid construct expressing only the mutant allele; AS-F, ASWT-R, ASΔE-R: allele-specific set of primers (described in Figure 1); Cp: values from PCR growth curves; ΔCp: Cp difference between the two allele-specific primers (Cp for wildtype minus Cp for mutant) for the same DNA template.