Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System
Figure 2
Labeling method for mature (a) and precursor (b) miRNA and their specific detection on ZIP-code array (c). Mature miRNA is labeled at the polyA-tail with Cy3-dUTP and unlabeled dTTP using miRNA-specific cZIP-Primer X; precursor miRNA is labeled at the consecutive precursor sequence with Cy3-dATP and unlabeled deoxynucleotides using miRNA-specific cZIP-Primer Y. The detection and differentiation of mature and precursor miRNA are based on the hybridization of the corresponding ZIP-code parts of the miRNA-specific cZIP-Primers to their complementary ZIP-code sequences immobilized at defined positions on the ZIP-code array.
