Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System
Figure 3
Exclusive detection of mature (a) and precursor (b) miRNA. Log signal intensities from three hybridizations of fluorescently labeled MSO-cZIPs with the ZIP-code array after specific labeling of synthetic miRNA templates are presented. (a) Specific labeling of mature synthetic miRNA templates with Cy3-dUTP and unlabeled dTTP using MSO-cZIP set A, and precursor synthetic miRNA templates were reverse transcribed with unlabeled deoxynucleotides and MSO-cZIP set B; (b) Specific labeling of precursor synthetic miRNA templates with Cy3-dATP and unlabeled deoxynucleotides using MSO-cZIP set B, and mature synthetic templates were reverse transcribed with unlabeled deoxynucleotides and MSO-cZIP set A. ZIPs for mature miRNA detection: A, C, E, G, I, L, N, P, and R; ZIPs for precursor miRNA detection: B, D, F, H, K, M, O, Q, and S.
