Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System

Figure 7

Detection of mature and precursor amiR in HeLa cells. Signal intensities from three hybridizations of fluorescently labeled MSO-cZIPs with the ZIP-code array after specific labeling are presented. Mature amiR was labeled with Cy3-dUTP and unlabeled dTTP using MSO-cZIP set A (gray), precursor amiR was labeled with Cy3-dATP and unlabeled deoxynucleotides using MSO-cZIP set B (black). ZIPs for mature miRNA detection: A, C, E, G, I, L, N, P and R. ZIPs for precursor miRNA detection: B, D, F, H, K, M, O, Q and S. The red line indicates the defined threshold value (190 a.u.).