Simultaneous Detection of Different MicroRNA Types Using the ZIP-Code Array System

Table 2

Sequences of ZIP oligonucleotides and miRNA-specific oligonucleotides-cZIP. Sequences of ZIP oligonucleotides (immobilized on DNA microarray) and miRNA-specific oligonucleotides with cZIP (miRNA-specific sequences are illustrated in bold).

Internal ZIP ID	MSO-cZIP set	Sequence of ZIP oligonucleotide on DNA microarray (5′ to 3′ orientation)	Sequence of miRNA-specific oligonucleotide-cZIP (MSO-cZIP) (5′ to 3′ orientation)
mi-9_ZIP A	A	GCAGTGCTCACCGTCCGCGA	TCGCGGACGGTGAGCACTGC TCATACAGCTAGATAACCAAAGA
mi-9_ZIP B	B	ACGCGACGCACCTGCTCCAA	TTGGAGCAGGTGCGTCGCGT TCATACAGCTAGATAACCAAAGA
mi-16_ZIP C	A	CGGAGTGGCACCAGCGGGAA	TTCCCGCTGGTGCCACTCCG CGCCAATATTTACGTGCTGCTA
mi-16_ZIP D	B	GTGGGAGCGACGCAGGGCAG	CTGCCCTGCGTCGCTCCCAC CGCCAATATTTACGTGCTGCTA
mi-92_ZIP E	A	TGGCGGTCTGCTGAGCGGTC	GACCGCTCAGCAGACCGCCA ACAGGCCGGGACAAGTGCAATA
mi-92_ZIP F	B	GCCTCGAGCCAACACCGCCT	AGGCGGTGTTGGCTCGAGGC ACAGGCCGGGACAAGTGCAATA
mi-100_ZIP G	A	TGGCCGGACAGGAGACACGC	GCGTGTCTCCTGTCCGGCCA CACAAGTTCGGATCTACGGGTT
mi-100_ZIP H	B	GCCTGCCTTCACGAGCCCAA	TTGGGCTCGTGAAGGCAGGC CACAAGTTCGGATCTACGGGTT
mi-19b_ZIP I	A	TGGCCGAGACTGCAGGAGCG	CGCTCCTGCAGTCTCGGCCA TCAGTTTTGCATGGATTTGCACA
mi-19b_ZIP K	B	ACGCCCTCCCAACCTCACGC	GCGTGAGGTTGGGAGGGCGT TCAGTTTTGCATGGATTTGCACA
mi-139_ZIP L	A	AGCGGACGACTGCGGACGAG	CTCGTCCGCAGTCGTCCGCT CTGGAGACACGTGCACTGTAGA
mi-139_ZIP M	B	TGGCGAGCGCAGTGGCAGAC	GTCTGCCACTGCGCTCGCCA CTGGAGACACGTGCACTGTAGA
mi-29a_ZIP N	A	AGCGTGCTGGTCGTGGGCCT	AGGCCCACGACCAGCACGCT TAACCGATTTCAGATGGTGCTA
mi-29a_ZIP O	B	AGCGCCAAGGGTCCTCGGGT	ACCCGAGGACCCTTGGCGCT TAACCGATTTCAGATGGTGCTA
mi-199b_ZIP P	A	GGGTGGTCCGGAGCGAGCAG	CTGCTCGCTCCGGACCACCC GAACAGATAGTCTAAACACTGGG
mi-199b_ZIP Q	B	CCTCAGACGGGTAGCGGCGA	TCGCCGCTACCCGTCTGAGG GAACAGATAGTCTAAACACTGGG
mi-99a_ZIP R	A	CCTCGTCCGTCCGGTCACCA	TGGTGACCGGACGGACGAGG CACAAGATCGGATCTACGGGTT
mi-99a_ZIP S	B	GGGTGTGGACGCGGAAGCAG	CTGCTTCCGCGTCCACACCC CACAAGATCGGATCTACGGGTT