Transcriptionally Repressive Chromatin Remodelling and CpG Methylation in the Presence of Expanded CTG-Repeats at the DM1 Locus
Figure 3
CTCF binds to CTCFbs1, also in the presence of expanded CTG-repeats. These graphs show enrichment ((Qt(IP)/Qt(input), normalised against the abChIP enrichment value of the positive control amplicon of each respective repeat length category) for CTCF in abChIP versus IgG-IP at three regions at the DM1 locus. CTCFbs1 shows statistically significant enrichment for CTCF in abChIP versus IgG-IP in all repeat length categories. No such CTCF-binding is seen at CTCFbs2 or at the enhancer region. Height of the bars indicates median enrichment; error bars indicate the interquartile range (25th to 75th percentile of observations). Mann-Whitney tests were performed to test for a statistically significant difference between the abChIP and IgG reactions. Results are summarised here with * being , ** being , and *** being . Details of the statistical analysis can be found in Supplementary Table 1. Enrichment values obtained for abChIP reactions were subjected to the Jonckheere Terpstra test for trend, which tests whether a trend exists across the categories with increasing CTG-repeat length. values are indicated in the graphs and details of this statistical analysis can be found in Supplementary Table 2.
